Glutamate: Glu: E: GAG: Glutamate: Glu: E: GGT: Glycine: Gly: G: GGC: Glycine: Gly: G: GGA: Glycine: Gly: G: GGG: Glycine: Gly: G: n/a: Aspartate or Asparagine: n/a: B: n/a: Glutamate or Glutamine: n/a: . Glutamate: Glu: E: Glutamine: Gln: Q: Glutamate or Glutamine: Glx: Z: Glycine: Gly: G: Histidine: His: H: Isoleucine: Ile: I: Leucine: Leu: L: Lysine: Lys: K: Methionine: Met: M: Phenylalanine: Phe: F: Proline: Pro: P: Serine: Ser: S: Threonine: Thr: T: Tryptophan: Trp: W: Tyrosine: Tyr: Y: Valine: Val: Amino-acid name 3-letter code 1-letter code Structure Properties; Alanine: Ala: A: Non-polar, aliphatic residues: Arginine: Arg: R: Positively charged (basic amino acids; non-acidic amino acids); Polar; Hydrophilic; pK=12.5: Asparagine: Asn: N: Polar, non-charged: Aspartate: Asp: D: Negatively charged (acidic amino acids); Polar; Hydrophilic; pK=3.9: Cysteine: Cys: C: Polar, non-charge Glutamate is a major constituent of a wide variety of proteins; consequently it is one of the most abundant amino acids in the human body. Glutamate is formally classified as a non-essential amino acid , because it can be synthesized (in sufficient quantities for health) from alpha-Ketoglutaric acid , which is produced as part of the citric acid cycle by a series of reactions whose starting point is citrate
Glutamine is an α-amino acid that is used in the biosynthesis of proteins. Its side chain is similar to that of glutamic acid, except the carboxylic acid group is replaced by an amide. It is classified as a charge-neutral, polar amino acid. It is non-essential and conditionally essential in humans, meaning the body can usually synthesize sufficient amounts of it, but in some instances of stress, the body's demand for glutamine increases, and glutamine must be obtained from the. Amino acid Letter code Side chain Hydropathy index Molar absorptivity Molecular mass Abundance in proteins (%) Standard genetic coding, IUPAC notation; 3 1 Class Polarity Charge, at pH 7.4 Wavelength, λ max (nm) Coefficient, ε (mM −1 ·cm −1) Alanine: Ala A Aliphatic Nonpolar Neutral 1.8 89.094 8.76 GCN Arginine: Arg R Basi Glutamate amino acid is technically a neurotransmitter but functions as an amino acid. It is also known as monosodium glutamate (MSG) DOI: 10.1007/s00726-012-1280-4. Abstract. Glutamate is one of the most abundant of the amino acids. In addition to its role in protein structure, it plays critical roles in nutrition, metabolism and signaling As a random constituent of protein, glutamate is the most abundant of all amino acid species in α-helices (aspartic acid is in the bottom third with respect to frequency and amino adipic acid is not a player because it is a post-translational amino acid). Glutamate is a principal contributor to the hydrogen bonding, hydration and coordination-chelation phenomena leading to the characteristic tighter packing of chain folds that permits α-helices to function as molecular springs
Valine and glutamic acid are amino acids with very different structures and properties. They are both building blocks of protein, and sometimes mutations in your DNA can cause substitution of one for the other. This can potentially lead to serious disorders, the most well-known of which is called sickle cell anemia G: Glycine: Gly: P: Proline: Pro: A: Alanine: Ala: V: Valine: Val: L: Leucine: Leu: I: Isoleucine: Ile: M: Methionine: Met: C: Cysteine: Cys: F: Phenylalanine: Phe: Y. Glutamate activates both ionotropic and metabotropic glutamate receptors. The ionotropic ones being non-NMDA (AMPA and kainate) and NMDA receptors. Free glutamic acid cannot cross the blood-brain barrier in appreciable quantities; instead it is converted into L-glutamine, which the brain uses for fuel and protein synthesis
D-glutamic acid is an optically active form of glutamic acid having D-configuration. It has a role as an Escherichia coli metabolite and a mouse metabolite. It is a D-alpha-amino acid and a glutamic acid.It is a conjugate acid of a D-glutamate(1-).It is an enantiomer of a L-glutamic acid . The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD Monosodium glutamate (MSG), also known as sodium glutamate, is the sodium salt of glutamic acid.MSG is found naturally in some foods including tomatoes and cheese. MSG is used in cooking as a flavor enhancer with an umami taste that intensifies the meaty, savory flavor of food, as naturally occurring glutamate does in foods such as stews and meat soups
, Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC; Glutamate: umol/L: 20642-5: 700068: Amino Acid Profile, Qn, Plasma: 35083-5: 700015: Glutamine: umol/L: 20643-3: 700068 Quantitative analysis of amino acid metabolism in liver cancer links glutamate excretion to nucleotide synthesis Avlant Nilssona,1 , Jurgen R. Haanstrab,1 , Martin Engqvista , Albert Gerdingc,d , Barbara M. Bakkerb,c , Ursula Klingmüllere , Bas Teusinkb , and Jens Nielsena,f,2 aDepartment of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden.
Scope /anticodon, /codon, /transl_except Contact EMBL Listing (note that the abbreviations are legal values for amino acids, not the full names) Abbreviation Amino acid name ----- ----- Ala A Alanine Arg R Arginine Asn N Asparagine Asp D Aspartic acid (Aspartate) Cys C Cysteine Gln Q Glutamine Glu E Glutamic acid (Glutamate) Gly G Glycine His H Histidine Ile I Isoleucine Leu L Leucine Lys K Lysine Met M Methionine Phe F Phenylalanine Pro P Proline Ser S Serine Thr T Threonine Trp W. Amino Acid Codes. The amino acid code that is used with the International Nucleotide Sequence Database is as follows. These amino acids are described with one letter abbreviation in /translation qualifier of CDS feature. The listed amino acid abbreviations are legal values for qualifiers /transl_except and /anticodon The simpler amino acids, Glutamate and Aspartate (just a simple carboxylate, nothing fancy) get first dibs on the easy-to-remember three letter codes (Glu, Asp). Glutamine and Asparagine's special characteristic is their amido group, so they get Ns in their three-letter codes (Asn, Gln)
code Three letter code Amino acid Possible codons; A: Ala: Alanine: GCA, GCC, GCG, GCT: B: Asx: Asparagine or Aspartic acid: AAC, AAT, GAC, GAT: C: Cys: Cysteine: TGC, TGT: D: Asp: Aspartic acid: GAC, GAT: E: Glu: Glutamic acid: GAA, GAG: F: Phe: Phenylalanine: TTC, TTT: G: Gly: Glycine: GGA, GGC, GGG, GGT: H: His: Histidine: CAC, CAT: I: Ile: Isoleucine: ATA, ATC, ATT: K: Lys: Lysine: AAA, AAG: L: Leu: Leucin High Blood Pressure Remedy Report. Natural High Blood Pressure Cure and Treatmen Amino acid: mRNA codons: Amino acid: mRNA codons: Ala/A: GCU, GCC, GCA, GCG: Leu/L: UUA, UUG, CUU, CUC, CUA, CUG: Arg/R: CGU, CGC, CGA, CGG, AGA, AGG: Lys/K: AAA, AAG: Asn/N: AAU, AAC: Met/M: AUG: Asp/D: GAU, GAC: Phe/F: UUU, UUC: Cys/C: UGU, UGC: Pro/P: CCU, CCC, CCA, CCG: Gln/Q: CAA, CAG: Ser/S: UCU, UCC, UCA, UCG, AGU, AGC: Glu/E: GAA, GAG: Thr/T: ACU, ACC, ACA, ACG: Gly/G: GGU, GGC, GGA, GGG: Trp/W: UGG: His/ In nervous system: Amino acids. Glutamate is the most abundant amino acid in the brain. Unlike acetylcholine, glutamate does not vary greatly in concentration from one region to the next. However, the dorsal gray matter of the spinal cord, which contains terminals of incoming dorsal roots, has large concentrations of Read Mor DB03425, 2s,4r-4-Methylglutamate DB01351, Amobarbital DB01352, Aprobarbital DB01483, Barbital DB00237, Butabarbital DB00241, Butalbital DB01353, Butobarbital DB01496, Dihydro-2-thioxo-5-((5-(2-(trifluoromethyl)phenyl)-2-furanyl)methyl)-4,6(1H,5H)-pyrimidinedione DB02852, Domoic Acid DB00142, Glutamic acid DB01354, Heptabarbital DB01355, Hexobarbital DB00463, Metharbital DB00849, Methylphenobarbital DB00312, Pentobarbital DB01174.
Start studying Amino Acid NT 1 - Glutamate and Aspartate. Learn vocabulary, terms, and more with flashcards, games, and other study tools Although CAT1 is the best characterized cationic AA transporter, other members have also been studied in the brain. CAT2 is expressed in the brain, but does not appear to be enriched at the BBB.
Now codons come in triplets which means that three letters will code for one amino acid. So take the first three letters in the strand GTT and find those on the codon table. You will see that it codes for Valine which would be the first amino acid in the sequence , to stand for the flavor enhancer, monosodium glutamate, but will now refer to the amino acid in monosodium glutamate that causes brain damage, endocrine disorders and adverse reactions, by its more factual name - Manufactured free Glutamate or MfG Glutamine (Gln), glutamate (Glu) and gamma-amino butyric acid (GABA) are essential amino acids for brain metabolism and function. Astrocytic-derived glutamine is the precursor of the two most important neurotransmitters: glutamate, an excitatory neurotransmitter, and GABA, an inhibitory neurotransmitter
. There are four of them, two basic amino acids, lysine (Lys) and arginine (Arg) with a positive charge at neutral pH, and two acidic, aspartate (Asp) and glutamate (Glu) carrying a negative charge at neutral pH Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:8123008, PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of.
Glutamate transporters are a family of neurotransmitter transporter proteins that move glutamate - the principal excitatory neurotransmitter - across a membrane.The family of glutamate transporters is composed of two primary subclasses: the excitatory amino acid transporter (EAAT) family and vesicular glutamate transporter (VGLUT) family.In the brain, EAATs remove glutamate from the. Deviant genetic codes reported in ciliates share the same feature: one (UGA) or two (UAR) of the three canonical stop codons are translated into one particular amino acid. In many genera, such as Oxytricha, Paramecium, and Tetrahymena, UAR codons are translated into glutamine. UGA is translated into However, there remains a possibility that two human-specific fixed amino acid substitutions, D71G in GRIN3A and R727H in GRIN3B, are related to human-specific brain function. Publication type MONOSODIUM GLUTAMATE. 8815. 6/5/2003 10:58:00 PM. Monosodium glutamate (MSG) is the sodium salt of the non-essential amino acid glutamic Glutamate is thus found in a wide variety of foods, and in its free form has been shown to. 2338
Valid for Submission. E72.19 is a billable diagnosis code used to specify a medical diagnosis of other disorders of sulfur-bearing amino-acid metabolism. The code E72.19 is valid during the fiscal year 2021 from October 01, 2020 through September 30, 2021 for the submission of HIPAA-covered transactions A cDNA has been isolated from a human brain expression library using anti-bovine glutamate dehydrogenase (GDH) antibodies. The cDNA has an open reading frame of 774 nucleotides, which codes for 258 amino acids. The 258-amino-acid sequence is 95% homologous to the carboxy terminus of human liver GDH What amino acid sequence does this code for? Solution. Use Figure 19.14 The Genetic Code to determine what amino acid each set of three nucleotides (codon) codes for. Remember that the sequence is read starting from the 5′ end and that a protein is synthesized starting with the N-terminal amino acid The nucleotide triplet that encodes an amino acid is called a codon. Each group of three nucleotides encodes one amino acid. Since there are 64 combinations of 4 nucleotides taken three at a time and only 20 amino acids, the code is degenerate (more than one codon per amino acid, in most cases). The adaptor molecule for translation is tRNA Alignment of predicted amino acid sequences for glutamate-gated chloride channels (TcGluCls) and γ-aminobutyric acid chloride channels (TcGABACls). N-terminal signal peptides are underlined. The positions of loops (LpA-F) involved in ligand binding, transmembrane motifs (TM1-4) forming the ion channel, and sites of cysteine residues are indicated by shadows
Nucleotide Codes, Amino Acid Codes, and Genetic Codes. Nucleotide codes. A : Adenine: G: Guanine: C: Cytosine: T: Thymine: U: Uracil: R: Purine (A or G) Y: Pyrimidine (C or T) N: Any nucleotide: W: Weak (A or T) S: Strong (G or C) M: Amino (A or C) K: Keto (G or T) B: Not A (G or C or T) H: Not G (A or C or T) D: Not C (A or G or T) V: Not T (A. The codon AUG codes for the amino acid methionine and serves as a start signal for the beginning of translation. Multiple codons may also specify the same amino acid. For example, the codons UCU, UCC, UCA, UCG, AGU, and AGC all specify the amino acid serine. The RNA codon table above lists codon combinations and their designated amino acids Glutamine (Gln) is found abundantly in the central nervous system (CNS) where it participates in a variety of metabolic pathways. Its major role in the brain is that of a precursor of the neurotransmitter amino acids: the excitatory amino acids, glutamate (Glu) and aspartate (Asp), and the inhibitory amino acid, γ-amino butyric acid (GABA) Metabolic Fate of Amino Acid Amino acid from ingested protein Cellular Protein Amino acid Alpha keto acid Alpha keto glutarate glutamate NH4 + Glutamine NH4 + Urea Uric Acid Liver 8. • Ammonotelic: Releases ammonia as excretory product. e.g., aquatic vertebrates, bony fishes and larvae of amphibia The amino acids glutamine and glutamic acid are closely related in a chemical sense. The human body is able to produce L-glutamine itself, from L-glutamic acid through the glutamate ammonium ligase. Considering the numerous metabolic processes glutamine is a part of, it is not surprising that it is the amino acid with the highest concentration in blood plasma, musculature and cerebral and.
Monosodium glutamate (MSG) is the sodium salt of the naturally occurring amino acid, glutamic acid which makes up 10 to 25 % of all food protein, from both animal and vegetable sources. In addition to being an ingredient intentionally added to foods, glutamate occurs as a natural part of proteins, including vegetable and animal proteins The antiporter system x c − imports the amino acid cystine, the oxidized form of cysteine, into cells with a 1:1 counter-transport of glutamate. It is composed of a light chain, xCT, and a heavy chain, 4F2 heavy chain (4F2hc), and, thus, belongs to the family of heterodimeric amino acid transporters Monosodium glutamate (MSG) is a salt of glutamic acid with molecular formula C 5 H 8 NO 4 Na. It is a white crystalline odourless powder, soluble in water. However, it is not hygroscopic. Glutamic acid is a non-essential amino acid which is synthesized by the body naturally SUPPORT | https://www.gofundme.com/ninja-nerd-scienceNinja Nerds,In this video we discuss transamination, and oxidative deamination along with the enzymes th.. Disorder of amino-acid metabolism, unspecified. 2016 2017 2018 2019 2020 2021 Billable/Specific Code. E72.9 is a billable/specific ICD-10-CM code that can be used to.
Anticodon Definition. Anticodons are sequences of nucleotides that are complementary to codons. They are found in tRNAs, and allow the tRNAs to bring the correct amino acid in line with an mRNA during protein production.. During protein production, amino acids are bound together into a string, much like beads on a necklace. It's important that the correct amino acids be used in the correct. The major route for amino acid loss is deamination and in general amino acids are only excreted by the kidney to a small extent. Glutamate can be found in the urine of humans (4.3 μg/mg creatinine in females) (Ragginer et al., 2012). Overall, glutamate is absorbed in the intestine and is metabolised to a great extent in the gut wall the usual G A G code tells the protein machine to put a glutamate amino acid from BIO 101 at Albany State Universit
Important in cellular metabolism, glutamate plays a role in memory and learning, and primarily functions and resides in the brain. Histidine. Three-letter code: His; One-letter code: H; An essential amino acid, histidine develops the myelin sheaths in the brain, which coat our nerve cells and allow for the transmission of electrical messages Glutamate amino acid synthesis >>> next page Text to speech synthesis When it comes to gun violence, christians too often either say nothing, i sent an essay to my editor, and received an enthusiastic e-mail back,
Find Glutamate Amino Acid stock images in HD and millions of other royalty-free stock photos, illustrations and vectors in the Shutterstock collection. Thousands of new, high-quality pictures added every day Start studying Amino acids. Learn vocabulary, terms, and more with flashcards, games, and other study tools 4 Key Amino Acids in Nitrogen Metabolism Glutamate o Important AA in amino acid from NUTR 3210 at University of Guelp (S)-Glutamic acid (L-Glutamic acid) is an endogenous, non-selective glutamate receptor agonist. S2862: VU 0364770. VU 0364770 is a positive allosteric modulator(PAM) of mGlu4 with EC50 of 1.1 μM, exhibits insignificant activity at 68 other receptors, including other mGlu subtypes. S2892: VU 036173 AMINO ACID SYNTHESIS Two amino acid transmitters glutamate Glu and gamma from PSYC 2240 at York Universit
This amino acid is found in a lot of different food including plant sources like soybeans, Glutamine is abbreviated as Q or Gln and is one of the twenty amino acids that the standard genetic code encodes. Glutamate. Glutamate is glutamic acid's salts and carboxylate anions Aminosäuren (AS), unüblich auch Aminocarbonsäuren, veraltet Amidosäuren genannt, sind chemische Verbindungen mit einer Stickstoff (N) enthaltenden Aminogruppe und einer Kohlenstoff (C) und Sauerstoff (O) enthaltenden Carbonsäuregruppe. Aminosäuren kommen in allen Lebewesen vor. Sie sind die Bausteine von Proteinen (Eiweiß) und werden frei bei der Zerlegung von Proteinen () Two amino acids have acidic side chains at neutral pH. These are aspartic acid or aspartate (Asp) and glutamic acid or glutamate (Glu). Their side chains have carboxylic acid groups whose pKa's are low enough to lose protons, becoming negatively charged in the process Q2: Glutamate is a key player in amino acid metabolism. 1) Describe all the pathways that glutamate participates in regarding amino acid metabolism. Be sure to include (key (a L-Glutamate side effects and Toxicity Glutamate causes neuronal damage and eventual cell death, particularly when NMDA receptors are activated, High dosages of glutamic acid may include symptoms such as headaches and neurological problems
other amino acids, including glutamate, with lower afﬁnity (K m. 300 mM) (5, 45, 46, 82, 93). The human neutral amino acid transporter ASCT1 is an obligate exchanger that does not mediate a net ﬂux of amino acids (111). Exchange of the amino acids is electroneutral and accompanied by a symmetrical ex-change of one Na1 Glutamate is an amino acid with very different functions: in the pancreas, it modulates the activity of the pancreatic ß-cells responsible for insulin production, whereas in the brain it is the. The X-ray crystal structure of the glutamate-GABA antiporter GadC is determined, revealing an inward-open conformation and providing insights into mechanism of amino acid antiport that is needed. A salt or ester of glutamic acid. g. acetyltransferase 1. an enzyme catalyzing transfer of an acetyl group from N2 acetylornithine to l g. forming l ornithine and N acetyl l g., an activator of the urea cycle Alanine is a simple amino acid which has just a methyl or CH3 group as its side chain. Since you see nothing but carbon and hydrogen, Alanine is a non-polar hydrophobic amino acid. It's important to recognize that this is a very small amino acid and capable of being 'wedged' into tight loops or chains. Valine Val
Many translated example sentences containing amino acid glutamate - Spanish-English dictionary and search engine for Spanish translations Any one who has studied the history of science knows that almost every great step therein has been made by the anticipation of Nature, that is, by the invention of hypotheses, which, though verifiable, often had very little foundation to start with; and, not unfrequently, in spite of a long career of usefulness, turned out to be wholly erroneous in the long run In fact, the abstract ends with the following quote: Our findings strongly support amino acid changes in hippocampus during seizures induced by pilocarpine, and suggest that glutamate-induced brain damage plays a crucial role in pathogenic consequences of seizures, and imply that strong protective effect could be achieved using lipoic acid through the release or decrease in metabolization. a, Schematic showing site-specific fluorescent labeling of mGluR2, with the unnatural amino acid 4-azido-L-phenylalanine at residue 548, by copper-catalyzed azide-alkyne click reaction Amino acids are organic compounds that contain amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.Amino acids play central roles both as building blocks of proteins and as intermediates in metabolism
L-Arginine L-glutamate salt Synonym: S(+)-2-Amino-5-guanidinopentanoic acid L-glutamate salt CAS Number 4320-30-3. Empirical Formula (Hill Notation) C 11 H 23 N 5 O 6. Molecular Weight 321.33 . EC Number 224-350-. MDL number MFCD00035566. PubChem Substance ID 2489101 Amino acid contents in sake and sake cake was quantified with an amino acid analyzer or an UF-Amino Station. 2.5. Whole genome sequencing analysis. The extracted genome DNA from the AZC-resistant strains (A901-8 and A902-4) and the parent strain K901 underwent quantification by Qubit (Thermo Fisher Scientific, Waltham, MA, USA)
The Single-Letter Amino Acid Code. G - Glycine (Gly) ; P - Proline (Pro) ; A - Alanine (Ala) ; V - Valine (Val) ; L - Leucine (Leu) ; I - Isoleucine (Ile) ; M. (4) Which amino acid is not chiral? Draw the structure. (5) Show the difference between the two negatively charged amino acids by drawing their structures. (6) What is the difference between glutamate and glutamine? Show by drawing the structure. (7) Which amino acid has a thiol group? Provide the full name, the 3-letter abbreviation and the. Glutamate receptor-like channels in plants: a role as amino acid sensors in plant defence? Brian G. Forde* and Michael R. Roberts Address: Lancaster Environment Centre, Lancaster University. (B) The R42W amino acid substitution in the mechanosensitive channel YfkC reduces intracellular glutamate levels. The intracellular metabolites of B . subtilis wild type strain 168, and the isogenic Δ yfkC , yfkC *, and yfkC * plsC * mutants were extracted and analyzed by GC-MS. (C) The R42W in the mechanosensitive channel YfkC results in an enhanced export of glutamate One of the two positively selected amino acid changes in the GLUD2 MTS involves a glutamic acid (E) to lysine (K) substitution at position 7 of the sequence (Figure 2A). Notably, this substitution-which occurred in the common hominoid ancestor during the time of the switch in targeting specificity-introduces a positive charge to the MTS by replacing a negatively charged residue ( Figure 2A )
Amino acid covariance in large sequence alignments has been shown to accurately predict amino acid physical proximity in 3D protein structures (Morcos et al., 2011; Hopf et al., 2012; Kamisetty et al., 2013; Nugent and Jones, 2012), and we used it as a proxy for coevolved inter-residu Glutamate decarboxylase (GAD, EC 18.104.22.168) is a unique pyridoxal enzyme that can catalyze α-decarboxylation of L-glutamate or its salts to produce γ-aminobutyric acid (GABA).Many neurological disorders, such as Huntington's chorea and Parkinson's disease, are associated with alterations of the GAD level because its substrate (L-glutamate) acts as an excitant of human neurons and its product. Glutamate is a multipurpose amino acid in the mature central nervous system. It is not only the major excitatory neurotransmitter, in addition it takes part in transamination and thus nitrogen homeostasis and is the precursor for other important molecules, including the main inhibitory neurotransmitter, γ-amino butyric acid (GABA)
The amino acids of the hemoglobin protein are represented as a series of linked, colored boxes. The lavender box represents the normal glutamic acid at position 6. The dark green box represents the valine in sickle cell hemoglobin. The other amino acids in sickle and normal hemoglobin are identical Glutamate, an amino acid that is found naturally in many foods that contain protein, is the main active component of monosodium glutamate. Although glutamate is found in many foods, it is often bound to other amino acids in protein molecules and, therefore, does not enhance flavor. Less often, glutamate is found in foods in its free, unbound form Glutamate has been shown to play a key role in the pathogenesis of brain ischemia (Dirnagl et al, 1999), and elevated concentrations of the amino acid have been reported in brain tissue surrounding the necrotic core both in animal models of ischemia (Benveniste et al, 1984; Hillered et al, 1989) and in stroke patients (Bullock et al, 1995; Kanthan et al, 1995), shortly after symptom onset